Let your USB drive plugged and run Autorun Eater or Flash Disinfector, allowing them to clean up all drives. They would create hidden folders named autorun.inf in each partition and every USB drive plugged in when you ran it. These folders protect your drives from future infection. After that, reboot your computer.
i think spybot is a really good antispyware 
Hi Tech,
Is it your recommended tools harmful to user?
They are not harmful to the user and are recommended to protect USB Flash sticks from infection.