First DOS-based malware celebrates silver jubilee
http://www.theregister.co.uk/2011/01/20/brain_virus_turns_25/
Congrats…!! ;D 8)
http://en.wikipedia.org/wiki/Brain_(computer_virus)
lol I like this part in the wikipedia article:
When the brothers began to receive a large number of phone calls from people in United States, United Kingdom, and elsewhere, demanding them to disinfect their machines, the brothers were stunned and tried to explain to the outraged callers that their motivation had not been malicious. They ended up having to get their phone lines cut off and regretted that [b]they had revealed their contact info in the first place[/b]. The brothers are still in business in Pakistan as Brain NET Internet service providers with a company called Brain Telecommunication Limited.
edit: yes I know some malware researchers do that officially, but that’s something else.
Btw, Elk Cloner was the first virus ‘in the wild’. (Written 1982 for Apple II.)
http://en.wikipedia.org/wiki/Elk_Cloner
asyn
Asyn,
also Virus 1,2,3 for Apple II (written 1981) 
CHK4BOMB, BOMBSQAD - first trojan horse detector by Andy Hopkins (1986)