Even local antivirus/antimalware programs will usually not scan files over 40MB-100MB in order to speed up scans and save system resources. Some programs allow you to raise this limit. With that said, I would try Comodo Cleaning Essentials (portable, can be run from a flash drive). In Options, raise the file size to 300MB, update the virus database and run a Custom scan on the file in question. CCE is very competent and I would trust it’s findings.

Direct download links:
http://download.comodo.com/cce/download/setups/cce_2.5.242177.201_x32.zip
http://download.comodo.com/cce/download/setups/cce_2.5.242177.201_x64.zip

PS You already have SAS. Enable file scanning over 4MB in Preferences > Scanning Control.